Deleted
Sample Discontinued
Experience: None
Not Used
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Part:BBa_K1732017:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)


pSB1C3-J23115-B0034-T7RNAP-B0034-YFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3640
    Illegal EcoRI site found at 119
    Illegal XbaI site found at 134
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 119
    Illegal NheI site found at 146
    Illegal NheI site found at 169
    Illegal SpeI site found at 3641
    Illegal PstI site found at 3655
    Illegal NotI site found at 125
    Illegal NotI site found at 3648
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 119
    Illegal XhoI site found at 4672
    Illegal XhoI site found at 5564
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 119
    Illegal suffix found in sequence at 3641
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 119
    Illegal XbaI site found at 134
    Illegal SpeI site found at 3641
    Illegal PstI site found at 3655
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence is codon optimized for E.coli


Source

Sequence designed by Cheryl Telmer from Bruchez lab in Carnegie Mellon University.

References